Biophysics PT. III: Strains in the cell

Created by Gabrielle Steppic

deformation
any change in distance between two material points of a continuous medium, representing the change in shape or size of an object under the effect of a force.

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TermDefinition
deformationany change in distance between two material points of a continuous medium, representing the change in shape or size of an object under the effect of a force.
how do deformations help study cells?they directly reflect how cells mechanically interact with their environment
biological examples of cell deformation- RBCs pass through a narrow capillary must undergo strong deformation - a cell moving through a dense matrix changes its shape to slip between surrounding fibers - the cell nucleus can compress or elongate in response to internal cytoskeletal forces
biological impacts of deformations- excessive nuclear deformations can cause nuclear envelope rupture = genomic instability - cancer cells actively modulate their ability to deform = better tissue invasion skills
deformations of cell migrationWhen a cell migrates through a tissue, it elongates in the direction of motion, emits protrusions (lamellipodia, filopodia), and generates traction forces via its cytoskeleton. These forces cause internal deformations visible at the cytoplasmic and nuclear levels.
material pointoccurs when a material deforms, each initial point of the material is moved to a new position -> then, the displacement vector is deformed
displacement vector depends on ___considered point; it defines a displacement field throughout the material equation : ⃗u = ⃗x − X⃗
displacement fielda vector function defined over the initial domain of the material
how can the displacement field be tracked how experimentally?through particle tracking, fluorescence microscopy imaging, or cell contour tracking
biological example of displacement fieldtraction force microscopy - a cell placed on a soft gel can exert tractions on it. mark the gel with fluorescent beads and track the displacements of the beads between the initial and deformed stations
mathematical modelling of traction force microscopyA bead is initially at position X⃗ = (10 μm, 5 μm) in a gel. After traction by a cell, it is observed at ⃗x = (12 μm, 6.5 μm). The displacement vector is therefore 2 μm horizontally and 1.5 μm vertically.
rigid displacement vs deformationrelative change between the object's points
uniaxial elongation deformationan object is stretched in one direction.
biological examples of uniaxial elongation- a cell pulled between two micropipettes - an actin fiber under tension.
compression deformationshortening in one direction.
biological examples of compression deformationnucleus compressed in a microchannel
shear deformationparallel sliding of layers
biological examples of shear compressiona cell subjected to fluid flow or on a soft substrate.
deformation in biology— Epithelial cells elongate during morphogenetic processes. — The cytoskeleton undergoes internal shear during migration. — Living tissues can be locally compressed by surrounding forces.
small strain tensor denoted ε. used to describe the local deformation of a material by expanding the displacement field around a given point
small, shape variation displacements can be approximated by ___first derivatives of displacement
symmetric tensormeasures the relative variation of distance between two neighboring points of the material.
elongations or contractions are measured withdiagonal components (εxx, εyy, εzz) along the axes
shear deformation is measured withoff-diagonal components (εxy), signifies changes in angles between initial axes
when a cell migrates on a soft substrate...it can locally stretch it - εxx > 0
a compressed cell nucleus experiences contraction in several directionsεii < 0
Xinitial position coordinates
xafter deformation coordinates
small strain tensor is symmetric, true or false?true
small strain tensors represent...the shape variation of the material
deformation gradient tensor signified by F describes how a small material volume transforms
if F = Ithere is no deformation
if F does not = I there is a significant local deformation of the material
biological examples of large deformation tensorIn tumors or embryonic tissues, cells exert significant traction on their environment (e.g., collagen, extracellular matrix). Large deformations of these matrices modify : — the distribution of forces around cells ; — the local tissue structure ; — the mechanical response of the cell via mechanosensitive sensors.
linear framework is no longer valid whendisplacements are large
deformation gradient tensor describes ___stretching, shear, and rotation of a small volume
deformation gradient tensor can be applied to cell migration, growth, tissue remodeling
deformation invariants certain quantities derived from the tensor that remain unchanged
when reference frame changes, ____ changestensor components
trace as a common invariant of small strain tensor trace -> tr(ε) = εxx + εyy + εzz represents the relative volumetric deformation positive = expansion, negative = compression
determinant of F (large deformations) as a common invariant of small strain tensordet(F) = local volume change ratio if det(F) > 1, the material has expanded if det(F) < 1, the material has contracted
biological example of strain tensor invariants During an osmotic shock, a cell swells or shrinks depending on the concentration gradient. The volume change can be described by : — the trace of ε if deformations are small ; — or det(F) if deformations are large.
what allows quantification of cytoplasmic or nuclear swelling from images?defining trace and the determinant of F in the small strain tensor
invariants are dependent or independent on the chosen reference frame?independent
the trace of ε measures what?the volume change (for small deformations)
the determinant of F gives what?local volumetric enlargement factor (non linear case)
biological applications of invariants?osmosis nuclear swelling growth