BIOL-101 Lab Microscopy

Created by R Whittin

Magnification
The amount an image is enlarged

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TermDefinition
Magnification
The amount an image is enlarged
Resolution/resolving power
The extent to which image detail is preserved
Contrast
The ability of an image to stand out against its background
Working Distance
Distance between objective & slide on stage
Field of view/diameter of field
Increase the magnification you decrease the field of view.
Parfocal
When you change lenses (to a higher or lower power) the image will always remain in focus.
Parcentral
When you switch objectives lenses the image will stay in the center of your field of view.
Light Microscopes
40x - 1000x Magnification
Light Microscopes
Produce a color image
Compound Light Microscope
Thin sections of specimen, light must pass through to see inside of the organism
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Stereo Dissecting Microscope
Light shines down on specimen to view outer surface detail
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Electron Microscope
350,000x Magnification
Electron Microscope
Produces black & white images (nonliving specimen only)
TEM
Transmission Electron Microscope
SEM
Scanning Electron Microscope
Scanning Power lens
4X
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Low Power lens
10X
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High Dry Power lens
40X
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Ocular Magnification
10X
Stage
holds slide
Stage clips
secure slide
Coarse adjustment
big focus (low power only)
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Fine adjustment
sharp focus
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Condenser
focuses light
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Diaphragm
controls light amount
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Light source
illuminator
Arm & base
support
Body
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