PHYSL 210A - RESPIRATORY

Created by Ameera Gani

Functions of resp system
- provide O2 and eliminate CO2 - protect against microbial infection - regulate blood pH - phonation - olfaction - reservoir for blood

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TermDefinition
Functions of resp system - provide O2 and eliminate CO2 - protect against microbial infection - regulate blood pH - phonation - olfaction - reservoir for blood
Components of the upper airways- nasal/oral cavities - pharynx - larynx
Components of the lungs- bronchi, bronchioles, alveoli - smooth muscle/connective tissue - pulmonary circulation
What are the trachea and primary bronchi made of - C-shape cartilage (anterior) - smooth muscle (posterior)
What are the bronchi made of plates of cartilage and smooth muscle
What are the bronchioles made ofsmooth muscle
What zones are the larynx divided into? describe them 1. conducting zone: leads gas to gas exchange region of lungs - "dead space" - no alveoli = no gas exchange 2. respiratory zone: where gas exchange happens - has alveoli
Terminal bronchioles- smallest airway without alveoli
Respiratory bronchioles- have occasional alveoli
Alveoli thin-walled capillary rich sac in lungs where O2 and CO2 exchange happens
Type 1 alveolar cells - lined by continuous mono-layer of flat epithelial cells - do not divide (susceptible to inhaled or aspirated toxins)
Type II alveolar cells- produce surfactant: detergent-like (lipoproteins), reduces surface tension of alveolar fluid - acts as progenitor cells: injury to type 1 cells causes type II cells to multiply and divide (eventually differentiate into Type I cells)
What type of transport is the transfer of O2 and CO2? where does it happen- occurs by diffusion - through the respiratory membrane (very thin)
What are the steps of respiration1. ventilation: exchange of air between atmosphere and alveoli by (bulk flow) 2. exchange of O2 and Co2 between alveolar air and blood in lung capillaries by (diffusion) 3. transport of O2 and CO2 through pulmonary and systemic circulation by (bulk flow) 4. exchange of O2 and CO2 between blood in tissue capillaries and cells in tissues by diffusion 5. cellular utilization of O2 and production of CO2
How is airflow produced 1. CNS sends rhythmic excitatory drive to resp muscles 2. resp muscles contract in pattern 3. changes in volume and pressure and chest and lungs 4. air flows in and out
Types of resp muscles- pump muscles: INS - diaphragm, external intercostals, parasternal intercostals EXP - internal intercostals, abdominals - airway muscles INS - tongue protruders, alae nasi, muscles around airway EXP - muscles are airways (pharynx, larynx) - accessory muscles INS: sternocleidomastoid, scalene
Diaphragm dome shaped muscle which flattens during contraction (INS), abdominal contents forced down and forward, rib cage widened - increased volume of thorax
External intercostal muscles contract and pull ribs upwards increasing volume of thorax - bucket handle motion
Parasternal intercostal muscles contract and pull sternum forward, increasing anterior/posterior dimension of rib cage - pump handle motion
Abdominals - external/internal oblique, transversus abdominis, rectus abdominis - relaxed at rest
Internal intercostal muscles - relaxed at rest - exercise: internal intercostal muscles pull rib cage down, reducing thoracic volume
Accessory INS muscles - scalenes: elevate upper ribs - sternocleidomastoids: raise sternum
What muscles contribute to opening upper airways and reducing airway resistance - tongue protruders - alae nasi - pharyngeal and laryngeal dilators (inspiratory) - pharyngeal and laryngeal constrictors (expiratory)
What is obstructive sleep apnea- reduction in upper airway patency during sleep - reduction in muscle tone - anatomical defects
What kind of cell is conducting airways lined with - epithelial cells which comprise mucus-producing and ciliated cells - entrap inhaled particulates and remove from airways
What do ciliated cells produce - periciliary fluid (sol layer)
What do macrophages in alveoli do phagocytize foreign particles
Spirometry- pulmonary function test - amount/rate of inspired and expired air
T/F: Residual volume cannot be measured by spirometryTrue
Atelectasiscomplete/partial collapse of lung (or lobe) - alveoli becomes deflated or collapsed
Tidal volume (TV) volume of air moved in/out during each ventilatory cycle
Inspiratory volume (IRV) additional volume of air that can be inspired following normal inspiration - maximum possible inspiration
Expiratory volume (ERV) additional volume of air that can be expired following normal expiration - maximum voluntary expiration
Residual volume (RV) volume of air remaining in lungs after maximal expiration
Vital capacities (VC) + formulamaximal volume of air forcibly inhaled after maximal inspiration VC = TV + IRV + ERV
Inspiratory capacity + formulamaximal volume of air forcibly inhaled IC = TV + IRV
Functional residual capacity + formulavolume of air remaining after normal expiration FRC = RV + ERV
Total lung capacity + formulavolume of air in lungs at the end of maximal inspiration TLC = FRC + TV + IRV = VC + RV
Total/minute ventilation total amount of air moved into resp system per minute "L/min"
Which is usually higher? alveolar or total ventilation per minutetotal
Alveolar ventilation formulaVa = (Vt - Vd) x frequency Vt = tidal volume Vd = dead space volume
T/F: Increase in breathing rate is best way to increase alveolar ventilationFalse, increased depth of breathing is the best way
Forced expiratory volume in 1 second (FEV1) - healthy person can blow most of their air out in 1 second
Forced vital capacity (FVC) - total amount of air blown out after max inspiration as fast as possible
Formula of the proportion of air that is blown out in 1 secondFEV1/FVC
What 3 patterns could the spirometry test show- Age, gender, weight, height (near high, just below restrictive %) - obstructive pattern (lower %) - restrictive pattern (high %) - Test will only show one
What does an obstructive pattern cause - shortness of breath (difficulty exhaling all air) - damage or narrowing of airways and lungs
What does a restrictive pattern cause- cannot fully fill lungs with air - stiffness in lungs - reduced vital capacity
Helium dilution method- insoluble in blood but equilibrates after a few breaths - conc measured after expiring V2 = V1 x (C1 - C2) / C2
What do static properties mean - mechanical properties when no air is flowing - needed to maintain lung and chest wall at a volume
What are the static properties - intrapleural pressure, transpulmonary pressure - static compliance of lung - surface tension of lung
What do dynamic properties mean mechanical properties when lung is changing volume
What are the dynamic properties- alveolar pressure - dynamic lung compliance - airway/tissue resistance
Boyles law- Pressure and volume and inversely proportional P1V1 = P2V2 (constant T) - gas molecules always in constant motion = pressure
Explain the formula for bulk flow, and state it F = Change in pressure (Palv - Patm) / Resistance - pressure difference between inside and outside lung moves air via bulk flow (F) from high pressure to low pressure
What happens to bulk flow (F) when Patm = PalvF = 0
Pleurae- double layered envelope - visceral: covers external surface of lung - Parietal: convers thoracic wall and superior face of diaphragm
Purpose of intrapleural fluidreduced friction of lung against thoracic wall during breathing
What does elastic recoil do to the lungstendency to collapse lungs
What does the chest wall do in relation to elastic recoilpulls thoracic cage outward
How do the lungs and chest wall interact through intrapleural space between visceral and parietal pleurae
Intrapleural pressure (Pip) - pressure in pleural cavity - ALWAYS subatmospheric bc of opposing directions of lungs and thoracic cage
What happens if Pip = Palv lungs collapse
Alveolar pressure - pressure inside alveoli
What point are Palv and Patm the samewhen glottic is open and no air goes in/out
Transpulmonary pressure (Ptp) + formula- force responsible for keeping alveoli open Ptp = Palv - Pip
Which pressure should be the highest Palv - then Pip - then Ptp (greater than 0)
Resistive forces - inertia - friction
T/F: If flow is laminar, airway resistance is more sensitive to changes in radiusFalse, more sensitive when flow is NOT laminar
T/F: transitional airflow increases resistance True, requires extra energy
T/F: the resistance is the lowest False, resistance is the highest
What does Poiseuille's law stateairway resistance is proportional to viscosity of the gas and length of tube
In disease conditions, do airways play larger or smaller role in airflow resistancelarger
Lung compliance- measure of elastic properties of lungs and how easily they expand - change in lung volume produced by a change in transpulmonary pressure
What is the slope of the pressure volume curve C = Change in volume (V) / Change in pressure (Ptp)
Static compliancelung compliance during periods of now gas flow - measured w/ P/V slope (end of expiratory event)
Dynamic compliance pulmonary compliance during periods of gas flow (inspiration) when transpulmonary pressure continuously changes
T/F: dynamic compliance is less than or equal to static lung complianceTrue
What causes dynamic compliance to fallincrease is lung stiffness or airway resistance
What do the first increases in VL reflect popping open of of proximal airways
What do low lung volumes mean at low lung volumes, it is difficult to pop open a collapsed airway
What happens when all airways are openPip becomes more negative and increases VL linearly
What happens at VLlung compliance decreases
Hysteresisdifference between inflation and deflation of compliance paths - greater pressure difference required to open a previously closed airway
Where are the elastic components of airways - localized in alveolar walls, around BV, and bronchi
Elastin- weak spring, low tensile strength, extansible
Collagen - strong twine, high tensile strength, inextensible
With aging, what happens with elastin and collagen decreases, lung compliance increases (floppy lungs)
Floppy lungs result of elastin and alveolar wall destruction
Pulmonary fibrosis collagen deposition in alveolar walls - reduction in lung compliance (stiff lungs) - high transpulmonary Pressure changes are necessary to generate changes in lung volume
What is lung compliance determined by - elastic components - surface tension at air-water interface within alveoli (ST decreases lung compliance)
Surface tensionmeasure of attracting forces acting to pull liquids surface molecules together at air-liquid interface
What causes alveolar collapse inward recoil due to alveolar surface tension
What does the smaller bubble's radius cause greater pressure to keep bubble inflated
T/F: in bubbles of different sizes, T remains constanttrue
What is alveolar sufactant purpose- produced by type II alveolar calls - lowers surface tension of lining fluid to breathe without effort - makes alveoli stable against collapse
What are the effects of hydrophobic and hydrophilic properties of surfactant cells - decreases density of water molecules
What does reduced surface tension of water mean for lungs increased lung compliance (easier to expand lungs)
Increase in SA means what for surfactantdecrease in thickness - increase in T (inside wall of alveoli) with increasing alveolar diameter
What does equalized pressure of diff size alveoli mean prevents collapse of small alveoli into large
T/F: premature infants are born w/ surfactant False, they lack it - decreases compliance (increased work of breathing)
How does the weight of the lungs effect Pip- increases pressure (less negative) - since alveoli are more deflated, they expand more (bottom regions of lung receive more inspired air)
Daltons lawin a mixture of gases, each gas operates independently - total pressure = sum of individual pressures
What does ficks law explain- rate of transfer of a gas through sheet of tissue is proportional to tissue area and gas partial pressure of the two sides - inversely proportional to the thickness
What does the diffusion constant determine- amount of gas transferred is proportional to gas solubility
T/F: oxygen more soluble that CO2False, CO2 faster
Henry's lawamount of gas dissolved is proportional to partial pressure of gas in which liquid is in eq
T/F: partial pressures are not the same in gase and liquidfalse, they are
Formula for conc of a gas (in a liquid) P x solubility
Why is the O2 pressure in air > O2 pressure in alveoli- warming increasing and humidifies air in resp tract = decreases pressure - loss of oxygen to blood diffusion = decrease - mixing of inspired air w/ functional residual volume = decrease
Determinants of oxygen pressure oxygen pressure in atm alveolar ventilation (Va)
Formula for alveolar ventilationVa = (Vt - Vd) x resp frequency
Determinants of alveolar CO2 pressure CO2 pressure in atm Va metabolic rate perfusion
What is the effect of increasing alveolar ventilation on PO2 and PCO2 PO2: increase PCO2: decrease
What is the effect of increasing metabolic rate on PO2 and PCO2PO2: decrease PCO2: Increase
Cardiac output volume of blood pumped by heart per min (mL blood/min)
Does systemic circulation require high or low pressure systemhigh - delivers blood in peripheral tissue
Does pulmonary circulation require high or low pressure systemlow - deliver blood to only lungs
Low pressure system - only needs to pump to top of lung - avoids rupture of resp membrane and edema formation
Low resistance system - R is less than 1/10 of that in systemic circulation due to short and wide vessels
High compliance vessels - higher # of arterioles w/ low resting tone - thin walls and paucity of smooth muscles = can accept lots of blood - can dilate in response to modest increase in arterial pressure
What makes up pulmonary circulatory system low pressure system, low resistance system, high compliance vessels
What is pulmonary BV450 mL
What is pulmonary capillary BV, at rest and exercise70mL at rest, up to 200 mL during exercise
Does blood move faster or slower through pulmonary capillaries when cardiac output increasesfaster (0.75 -> 0.3 seconds)
Explain the ventilation/perfusion ration (V/Q) - balance between ventilation (bringing in O2 and removing CO2 from alveoli) and perfusion (removing O2 and adding CO2 into alveoli)
What happens to PCO2 and PO2 with greater ventilation - the more closely they approach their values in inspired air
High V/Q ratioalveolar dead space
Alveolar Vdregions of lung w/ high V/Q ratio - overventilated (underperfused) - portion of fresh air is reach alveoli that cannot be taken up by blood
Anatomical Vdvolume of conducting airways that dont participate in gas exchange
Low V/Q ratioairway obstruction
Shuntportion of venous blood doesnt get oxygenated and goes back to arterial blood - associated with low V/Q ratio
Local ventilation-perfusion ratio(Va/Q) - local alveolar PO2 and PCO2
In an upright patient, perfusion is greatest where in the lungs?- greater near the base of the lung and falls towards apex (top of lung)
What forces does perfusion depend on - gravity - posture
Compared to basal lung, is BFlow and alveolar ventilation reduced or increased in apical lung reduced
What is the ideal basal VA/Q0.6
What is the ideal apical Va/Q0.3
Why does pulmonary hypoxic vasoconstriction occur response to low O2
O2 is carried in blood by what 2 ways 1. dissolved (minor) 2. combined w/ hemoglobin (major)
What law does dissolved O2 followHenry's law - O2 content is proportional to pressure of O2 and solubility
What 4 amino acids is Hb composed of - globins (2 alpha 2 beta) - 4 heme groups
What structure does each HEME ring has poryphrin - contains an iron in ferrous (Fe2+) form, O2 binds
What 2 ways can Hb exists as- deoxyhemoglobin - oxyhemoglobin
Explain the axis meanings of the oxygen dissociation curve x: PO2 in blood y: % of Hb binding sites that have Hb attached
O2 capacity max amount of O2 that can be combined w/ O2
Hb saturation% of available Hb binding sites that have O2 attached
Formula for Hb saturationO2 combined w/ Hb / O2 capacity x 100
What are the determinants of Hb saturation- arterial PO2 (crucial) - dissociation curve: sensitive to..pH, PCO2, Temp - Cooperative binding: = sigmoidal dissociation curve
Cooperative binding - O2 binds to heme group, deforms shape of heme group - changes shape of globin chain from tense to relaxed state
What does the change in shape of one globin chain do to the rest - deforms the others = exposes iron in heme group = more O2 binding
2 portions of the sigmoidal dissociation curve 1. flat (plateau): between 60-100 mmHg 2. steep: 10-60 mmHg
What does the plateau mean for saturation stays high over wide range of alveolar PO2 - safety factor: significant limitation of lung function still allows normal O2 saturation of Hb
What does the steep portion demonstrate large amounts of O2 only lead to small decreases in PO2
Why is it important that PO2 remains high in peripheral tissue - to drive diffusion from RBC to blood cells and mitochondria
What do small changes in the dissociation curve cause on O2increase unloading
At rest, what is most Hb saturation leaving peripheral tissues75% saturated
What effect does steep portion demonstrate on metabolic rate- increases it, decreases PO2 in tissue = drop in plasma PO2, diffusion of O2 from RBC, drop in PO2 in RBC
Anemia vs. PolycythemiaAnemia: reduced Hb Polycythemia: increased Hb or decreased BV
Does carbon monoxide increase or decrease O2 unloading to tissueDecrease, CO has more affinity for Hb
What is the PO2 alv before diffusion vs after diffusion in resp membrane Before: PO2 alv > PO2 blood At eq: PO2 alv = PO2 blood
Does O2 - Hb contribute to PO2 value No
What is the PO2 alv before diffusion vs after diffusion in peripheral tissuePO2 Blood > PO2 interstitial fluid > PO2 cell > PO2 Mitochondria
What does a reduction in PO2 cause reduced affinity of O2 for Hb and more O2 released from RBC
What does a change to right mean for O2 dissociation curveO2 affinity of Hb is reduced = more unloading
What does a change to left mean for O2 dissociation curveO2 affinity for Hb is increased = less unloading
What is DPG and what are its effects on O2 dissociation curve- end product of metabolism - shifts curve to right
What could high levels of DPG causechronic hypoxia
In what 3 forms in CO2 carried in blood - dissolved - bicarbonate - carbamino compounds
T/F: CO2 solubility is lowfalse
Carbonate form of CO2 Carbonic anhydrase (CA) in RBC
What does CA do + effect on HCO3 Converts CO2 + H2O -> H2CO3 - this causes HCO3 to exit cells to maintain neutrality - H+ increases in venous blood (lowers pH)
What is a carbamino compound + rxn- combo of CO2 w/ amino group in blood proteins (globins in Hb) - Hb + CO2 -> HbCO2
Which has higher affinity for CO2, deoxy or oxyHb deoxyHb - CO2 helps w/ unloading in peripheral
Explain CO2 movement in peripheral tissue - CO2 exits cells dissolved in interstitial fluid and diffuses to blood - remains in plasma as dissolved CO2 (PCO2) - enters RBC and remains dissolved as CO2 - bound to deoxyHb or reacts w/ water to produce HCO3 and H (HCO3 exits RBC, H interacts w/ Hb)
What is PCO2 alv before diffusionPCO2 alv < PCO2 blood - dissolved CO2 in blood diffuses to alveoli - PCO2 in plasms recalls dissolved CO2 from RBC and change eq for CO2/H2O and CO2/Hb rxn
Is eq the same for tissues and lungsno, in the lungs the CO2 eq is reversed - H interacts w/ HCO3 and Hb can bind w/ O2
Respiratory acidosis- hypoventilation (CO2 production > CO2 elimination), PCO2 increases and H conc increases
Respiratory alkalosis- hyperventilation (CO2 production < CO2 elimination), PCO2 decreases and H conc decreases
Metabolic acidosis- increases H conc in blood
Metabolic alkalosis- decrease H conc in blood
In what system is breathing established in CNS - initiated in medulla by specialized neurons
Is breathing modified by higher or lower structures of CNS- higher - input from central and peripheral chemoreceptors and mechanoreceptors in lung and chest wall
What are the respiratory neurons in the brainstem - pontine resp group, dorsal resp group, ventral resp group
Prebotzinger complex- group of neurons in ventral resp group - generates excitatory inspiratory rhythmic activity which excites inspiratory muscles
Which pathway do the PreBotC and pFRG takepolysynaptic pathway
Parafacial resp group - group of neurons in ventral resp group - generates excitatory active expiratory rhythmic activity which excites expiratory muscles
Why does rhythym of breathing need to change- metabolic demands (changes in blood) - mechanical - non-ventilatory behaviour - pulmonary/non pulmonary diseases
Neuro resp pathway: inspirationpreBotC: INS premotorneuron (rostral ventral group) -> phrenic/thoraic motorneurons (in cervical/thoracic spinal cord) -> diaphragm and intercostal muscles preBotC: premotorneuron (rostral VRG and parahypoglossal region) -> cranial motorneurons (in medulla) -> tongue & upper airway muscles
Neuro resp pathway: expiration pFRG: premotorneurons (caudal VRG) -> thoracic & lumbar motorneurons (in spinal cord) -> intercostal & abdominal muscles
What are the effects of hypoxia (low PO2), hypercapnia (high PCO2), and acidosis (Low pH) on ventilationcauses increase - raises PO2, lower PCO2, increase pH
What do chemoreceptors do in controlling ventilation sense changes in PO2, PCO2, pH
Purpose of carotid and aortic bodies - sense hypoxia (low artial PO2) and sensitive to pH
Types of carotid body cellsType I: glomus cell - chemosensitive cells Type II: sustentacular cell - act as support in carotid body
Glomus cells- voltage gated ion channels - have many vesicles w/ neuroT's
What is the primary stimulus for peripheral chemoreceptors- decrease in arterial PO2
Glomus cells response to decrease in PO2- increase firing rate of AP
At what arterial PO2 does stimulation of chemoreceptors occur at below 60 mmHg - flat at 60-120 mmHg
How do peripheral chemoreceptors control resp muscles - activate dorsal and ventral resp groups in medulla - increase resp rate - increase tidal volume
Where are central chemoreceptors locatedclose to ventral surface of medulla (close to CSF and blood vessels) - rostral, intermediate, caudal regions
What receptors are responsible for hypercapniacentral chemoreceptors
Why does H stimulate mostly peripheral chemoreceptors because H does not cross BBB (CO2 does)
What effect does strenous exercise have on ventilationlactic acid buildup -> peripheral chemoreceptors -> hyperventilation