Cheek Cell DNA Extraction (LAB)

Created by Kalee

What is DNA? What do the letters DNA stand for? Make sure you can spell it.
DNA stands for Deoxyribonucleic Acid. It is a molecule that stores genetic information and contains instructions for an organism's growth, development, and functions. Spelling: De-ox-y-ri-bo-nu-cle-ic acid

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TermDefinition
What is DNA? What do the letters DNA stand for? Make sure you can spell it.
DNA stands for Deoxyribonucleic Acid. It is a molecule that stores genetic information and contains instructions for an organism's growth, development, and functions. Spelling: De-ox-y-ri-bo-nu-cle-ic acid
What is the basic DNA structure? what is the backbone?
DNA has a double helix structure, which looks like a twisted ladder. The backbone is made of alternating deoxyribose sugars and phosphate groups, while nitrogenous bases (A, T, C, G) form the steps of the ladder. Remember: Sugar + Phosphate = DNA backbone. đź’–
what are the rungs of the DNA ladder?
The rungs (steps) of the DNA ladder are made of nitrogenous base pairs held together by hydrogen bonds. - Adenine (A) pairs with Thymine (T). - Cytosine (C) pairs with Guanine (G). Remember: A pairs with T, and C pairs with G.
What are the base pairs of DNA?
A: The base pairs of DNA are: - Adenine (A) pairs with Thymine (T). - Cytosine (C) pairs with Guanine (G). Remember: A–T and C–G.
How do the base pairs match up to each other to create the double strand? (which nitrogen bases form hydrogen bonds)
A: DNA bases pair through hydrogen bonds to hold the two strands together. - Adenine (A) pairs with Thymine (T) using 2 hydrogen bonds. - Cytosine (C) pairs with Guanine (G) using 3 hydrogen bonds. Remember: A–T = 2 bonds, C–G = 3 bonds.
DNA Function?
DNA stores genetic information and provides instructions for making proteins that determine an organisms characteristics and functions.
Why is DNA considered your “genetic essence”?
DNA contains your genetic information, which determines your inherited traits and makes you genetically unique.
What is precipitation?
Precipitation is the process of separating a substance from a solution by forming a solid.
What is DNA spooling?
DNA spooling is the process chess of collecting DNA by winding its strand around an object, such as a plastic hook or glass rod (Think about what you did in lab that day as well).
Why was the test tube placed in a water bath of 80 degrees?
To help the detergent break down the cell and nuclear membrane faster, releasing the DNA.
What was the purpose of the powerade?
To collect cheek cells by swishing the Powerade around the mouth, allowing cells to loosen and enter the solution.
What was the purpose of the detergent?
To break down the cell and nuclear membranes, allowing the DNA to be released.
What was the purpose of the ethanol?
To precipitate the DNA, separating it from the solution so it becomes visible and can be collected.
What type of cells did the DNA come from?
The DNA came from cheek epithelial cells, which line the inside of the mouth.
What other type of nucleic acid could have been in your sample? Why/where would it come from?
RNA (ribonucleic acid) could also be present because it is naturally found in cheek cells and helps with protein production.