PHYSL 210A - REPRODUCTION

Created by Ameera Gani

What is the purpose of the seminiferous tubules? 1
Produce sperm

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TermDefinition
What is the purpose of the seminiferous tubules? 1
Produce sperm
What do leydig cells do? what type of cell are they? 2
- Secrete testosterone - Interstitial cells
What do sertoli cells do? what type of cell are they? 2
- Support sperm development - Epithelial cells
What do smooth muscle cells do? what type of cell are they? 2
- Peristalsis (propel sperm) - Myoid cells
What comprises the blood-testis barrier? 3
- Tight junctions - Luminal compartment - Basal compartment
What does "tropic role" mean?
Support sperm development
T/F: myoid cells secrete luminal fluid for sperm housing
False, sertoli cells do this
Sertoli cells: What is the androgen-binding protein? 2
- Androgen buffer - Maintain steady conc of Androgen in lumen
What do sertoli cells do in relation to testosterone and FSH? 2
- Act as target cells for those hormones - Secrete paracrine factors which stimulate spermatogenesis
Sertoli cells: what does inhibin do?
- Secreted by sertoli cells - Hormone for negative feedback loop of FSH
T/F: Sertoli cells phagocytize old sperm
True
Where is the site of immunosuppression?
Sertoli cells (Blood testis barrier)
What are the traits of adolescence? 2
- Up to age 10-14 - Unable to reproduce
What are the traits of puberty? 3
- Usually at age 10-14 (later in boys) - Sexual maturation (can produce gametes) - Secondary sexual traits develop
What are secondary sexual characteristsics? 3
- Hair - Voice - Height
Can androgens stimulate spermatogenesis? Can they stimulate sex drive?
Yes
T/F: Androgens do not promote secondary sexual traits?
False, they do
What is the anabolic effect?
Promote protein synthesis in skeletel muscle
What stimulates growth hormone secretion?
Androgens
What are the 3 main phases of spermatogenesis?
- Mitosis: ensures supply of spermatogonia - Meiosis: half the chromosome number - Differentiation and packaging: transport genetic material
Where does spermatogenesis occur? 2
- From basement membrane to lumen - Occurs in space between adjacent sertoli cells
Where does mitochondria occur in sperm?
Midpiece
What is the sperm head made of? 2
- Nucleus (chromosomes) - Acrosome (enzymes for fertilization)
Where is spermatozoa released into?
Lumen of semineferous tubules
How does fluid from sertoli cells go to epididymis?
- Pressure from cells
How does sperm and fluid go to vas deferens?
Peristalsis
LH vs FSH
LH: androgen secretion FSH: spermatogenesis
What are functions of seminal fluid? 3
- Dilute sperm - Provide energy (fructose) - Form semen clot
What are the accessory glands? 3
- Seminal vesicles - Prostate gland - Bulbourethral glands
Purpose of seminal vesicles
Secrete alkaline fluid with fructose, enzymes, prostoglandins
Purpose of prostaglandins
- Secrete citrate and enzymes
Purpose of bulbourethral glands
Secrete viscous fluid w/ mucus
T/F: male response controlled by CNS
False, its by ANS
What are the phases of the male sexual response? 3
- Erection: parasympathetic - Emission: sympathetic stimulation to smooth muscles of ducts - Ejaculation: contraction of skeletal muscle for semen release (somatic control)
Menarche
Onset of puberty for females
Menstruation
Female reproductive system cycles
Menopause
Lose ability to reproduce around 45-50
What are the female reproductive organs? 2
- Gonads (ovaries) - Reproductive tract (uterus, uterine tuber, vagina)
T/F: Ovaries are separate from reproductive tract
True
Purpose of uterine tubes
Transport ova from ovaries to uterus
Purpose of infundibulum and fimbriae 2
- Pick up released ovum - Move/release ovum through uterine tubes
What is the outer layer of the uterus?
Perimetrium - Epithelial cells and connective tissue
What is the middle layer of the uterus?
Myometrium - Thick - Smooth muscle
What is the inner layer of the uterus?
Endometrium - Layer of epithelial cells - Layer of connective tissue - Glands
What is the cervix?
Canal leading to vagina
What is the birth canal?
Cervix + vagina
What is the female gamete?
Egg, or an oocyte
How many oocytes are in 1 follicle during primary oocyte stage?
One
What is a primordial follicle?
- Oocyte - Layer of specialized epithelial cells
What are granulosa cells?
Layer of epithelial cells divides to become more than 1 layer
What is folliculogenesis?
- Granulosa cells process through zona pellucida and form gap juntions - Nutrients/paracrine factors sent through junctions to help oocyte growth
What is FSH's effect on granulosa cells? and estrogen?
- Stimulate cell multiplication - Produce estrogen
What type of receptors do theca cells have?
LH receptors
What does LH do to theca cells?
Stimulate androgen secretion
What do androgens do to granulosa cells? What does it lead to for estrogen?
- Permeate to cells and convert to estrogen in granulosa cells - Estrogen secretion rises and dominant follicle selected
Functions of granulosa cells 6
- Secrete antral fluid - Provide nutrients for oocyte - Secrete paracrines for follicle - Secrete inhibin - Secrete estrogens - Secrete substances that forms zona pellucida
What is the pulse generator?
Frequency and amp of pulses change every 24hr of menstrual cycle
Is number of oogenia for females fixed or continually producing?
Fixed at birth
When does mitosis start in embryonic life?
In first 2 months
When does meiosis 1 begin?
In fetal life
When does meiosis 1 stop?
Through birth until puberty
When does 1st meiotic division complete in dominant follicle?
Prior to ovulation
When does meiosis II complete?
After fertilization
What is the net result of Oogenesis?
1 primary oocyte produces 1 egg (n)
What is the net result of spermatogenesis?
1 primary spermatocyte produces 4 spermatozoa (n)
Are ovarian hormones required for female differentiation before birth?
No
What graph do ovarian hormones regulate?
Hypothalamic-pituitary gonadal axis
What are the components of the hypothalamic-pituitary gonadal axis? 3
E: Both + and - feedback effects P: - feedback only Inhibin: - feedback on FSH
What do low plasma levels of estrogen cause? When?
Negative feedback early to mid follicular phase
What do high levels of estrogen cause?
LH surge during late follicular phase
What happens during the menstrual phase? 4
- Shed uterine lining - BF to tissue decreases - Dead tissue and fall into vagina = flow - Hormones: due to decreased E/P Hormones decrease when corpus luteum degenerates
How does the uterus prepare for fertilized ovum during proliferative phase? 5
- Endometrial lining develops - Endometrial layer grows - Endometrial glands enlarge - Smooth muscle thickens (myometrium) - Cervical glands secrete mucus
What do estrogens do to uterine lining?
Stimulate development of it
How does the endometrium prepare for implantation during secretory phase? 3
- Increased blood supply - Glands enlarge and secrete glycogen-rich fluids - Cervical secretions more sticky = plug
What is to product of the fusion of male and female gametes?
Zygote
How long are the sperm and oocyte viable for fertilization?
Sperm: 5 days Oocyte: 12-24 hrs
What does the block to polyspermy do? 4
- Change membrane potential - Release contents of cortical granules - Enzymes enter and harden zona pellucida - Enzymes inactivate sperm binding receptor
What does the fusion of sperm to oocyte mean for division?
Stimulates 2nd meiotic division of oocyte Oocyte -> ovum
What happens to sperm plasma membrane during fertilization?
Disintegrates
What happens to DNA during fertilization?
Replicated -> zygote
What are mitotic divisions called?
Morula
How do you get identical twins?
Division of totipotent morula cells
How do you get fraternal twins?
Fertilization of two oocytes (during same cycle)
What does the outer cell layer of a blastocyst become? what is it called?
- Becomes fetal placenta - Trophoblast
What does the inner cell mass of a blastocyst become?
Becomes embryo
What is the fluid filled cavity of a blastocyst called?
Blastocoele
T/F: By 5 weeks, the placenta is functioning and heart is beating
True
Functions of the placenta 3
- Endocrine organ - Exchange tissue - Immunity/filter
What are the 4 major hormones of pregnancy?
- hCG: Human chorionic gonadotropic - hCS: human chorionic somatomammotropin - Progesterone - Estrogen
Purpose of hCG? 1
Maintain luteum functions in early pregnancy
Purpose of hCS? 2
- GH like and anti-insulin actions in mother - Helps fetus get more glucose
Purpose of progesterone? 4
- Decrease contractions - Inhibit LH and FSH - Grow mammary alveolar glands - Secrete sperm unfriendly mucus
Purpose of estrogen? 3
- Growth of uterus - Grow mammary ducts - Inhibit LH and FSH
What does the mammary gland look like from birth to puberty?
A few ducts is any alveoli
What does the mammary gland look like at puberty?
- Ducts grow/branch - Alveolar growth - Fat deposit around alveolar tissue
What does the mammary gland look like during pregnancy and lactation?
- Prolactin - hPL - Oxytocin
What is the purpose of PRL? prolactin
Lactogenesis - Initiation of milk synthesis - Low estrogen and progesterone
What is the purpose of hPL?
Growth factors
What is the purpose of oxytocin?
Milk ejection
What is galactopoiesis?
Maintenance of lactation - Needs PRL
What are the three factors to determine the sex of a person?
- Genetic: type of sex chromosome - Gonadal: Ovaries/testes - Genitgal/phenotypic: type of internal and external genetalia
T/F: chromosomal (genetic sex) is determined in 2nd trimester
False, its determined at fertilization
What dictates differentiation of bipotential gonads to become testes?
Presence of SRY gene on Y chromosome
What does XY dictate?
Male, testes develop
What does the presence of more than one X chromo mean?
Impaired spermatogenesis
What is the chromosomes of someone w/ Klinerfelters syndrome?
XXY Male
What is Turners syndrome?
Lack of X chromos
T/F: XXY males are fertile
False
In males, what do the testes secrete from sertoli cells?
MIH
What does MIH do?
Cause regression of Mullerian duct and regression of internal female genitalia
What do testes secrete from Leydig cells?
Testosterone
What is testosterone converted to?
DHT
What does DHT do?
Causes masculinization of male genitalia
In females, what does the absence of MIH do?
Allow mullerian duct to grow into female internal genitalia
What does the absence of testosterone do? for females
Prevent development of Wolffian duct = no male internal genitalia
Sexual disorders: Female XX but outwardly male
Congenital adrenal hyperplasia
What happens to hormones during Congenital adrenal hyperplasia?
Decreased cortisol = increased ACTH to adrenal androgens to masculrize external genitalia
Sexual disorders: Male XY but outwardly female
Androgen sensitivity syndrome
What happens during androgen sensitivity syndrome?
No internal ducts develop - breasts develop - Androgens converted into E targeted tissues - Infertile
What triggers the onset of puberty?
Increased secretion of GnRH from pulse generator -> increased LH/FSH -> increased sex steroids -> puberty changes
How does follicular depletion happen?
Apoptosis
What does decreased follicle #'s and decreased responsiveness to gonadotrophs do to E?
Decreased E and inhibin
What does no ovulation mean for progesterone?
No corpus luteum = decreased progesterone
What does decreased E, inhibin, and P do to FSH and LH?
Removes neg feedback inhibition = increased FSH/LH